Protein Production Service
Time frames
A. HEK-293 Mammalian System
Feature: Best choice for expressing almost any protein, with best success rate for secreted proteins
| Phases | Time |
| Expression vector construction | 1 week |
| Amplification of recombinant Plasmid | 1 week |
| Protein production | 1 week |
| Purification | 1-2 weeks |
| Total | 4-5 weeks |
B. Bacterial System
Feature: Cost effective; protein size up to 250 kDa
| Phases | Time |
| Expression vector construction | 1-2 weeks |
| Generation and selection of bacterial colonies expressing target protein | 1-2 weeks |
| Culture of recombinant bacteria | 2 days |
| Purification | 2-3 weeks |
| Total | 4-7 weeks |
C. Baculoviral System
Feature: Protein expressed in this system lacks most post-translational modifications; nearly unlimited protein size
| Phases | Time |
| Expression vector construction | 1-2 weeks |
| Generation and selection and plaque purification of baculoviral colonies expressing target protein | 2-3 weeks |
| Amplification of recombinant baculovirus; generation of high-titer virus stock | 1 week |
| Protein production | 1 week |
| Purification | 1-2 weeks |
| Total | 5-9 weeks |
D. Yeast System
Feature: Cost effective
| Phases | Time |
| Expression vector construction | 1-2 weeks |
| Generation and selection of yeast colonies expressing target protein | 1-2 weeks |
| Protein production | 1 week |
| Purification | 2-3 weeks |
| Total | 5-8 weeks |
Product Catalog
| Service description | Catalog # | Price |
| Set-up fee (includes the cost for constructing the expression vector and testing the protein expression) | GI-P100 | Please inquire |
| Mammalian/Baculoviral System: 1mg | GI-P110-1MG | Please inquire |
| Mammalian/Baculoviral System: 1-5mg | GI-P110-5MG | Please inquire |
| Mammalian/Baculoviral System: 5-10mg | GI-P110-10MG | Please inquire |
| Mammalian/Baculoviral System: 10-20mg | GI-P110-20MG | Please inquire |
| Mammalian/Baculoviral System: >20mg | GI-P110-C | Please inquire |
| Bacterial/Yeast System: 1mg | GI-P120-1MG | Please inquire |
| Bacterial/Yeast System: 1mg | GI-P120-1MG | Please inquire |
| Bacterial/Yeast System: 1-5mg | GI-P120-5MG | Please inquire |
| Bacterial/Yeast System: 5-10mg | GI-P120-10MG | Please inquire |
| Bacterial/Yeast System: 10-20mg | GI-P120-20MG | Please inquire |
| Bacterial/Yeast System: >20mg | GI-P120-C | Please inquire |
Guaranteed >95% purity for secreted protein and >75% purity for intracellular proteins
Contact us
If you like to purchase, or need any assistance, please do not hesitate to contact us at:
20501 Seneca Meadows Parkway
Suite 300-F
Germantown, MD 20876
Tel. 301-861-4366
Order: order@geneimmuneusa.com
Info: info@geneimmuneusa.com