Protein Production Service

GeneImmune offers customer protein production service with quality and flexibility in mind. Our choices of expressing systems: mammalian cells, baculovirus, yeast, and E.coli will give our customers great flexibilities to cost-effectively meet their needs.

Time frames

A. HEK-293 Mammalian System

Feature: Best choice for expressing almost any protein, with best success rate for secreted proteins

Phases Time
Expression vector construction 1 week
Amplification of recombinant Plasmid 1 week
Protein production 1 week
Purification 1-2 weeks
Total 4-5 weeks

B. Bacterial System

Feature: Cost effective; protein size up to 250 kDa

Phases Time
Expression vector construction 1-2 weeks
Generation and selection of bacterial colonies expressing target protein 1-2 weeks
Culture of recombinant bacteria 2 days
Purification 2-3 weeks
Total 4-7 weeks

C. Baculoviral System

Feature: Protein expressed in this system lacks most post-translational modifications; nearly unlimited protein size

Phases Time
Expression vector construction 1-2 weeks
Generation and selection and plaque purification of baculoviral colonies expressing target protein 2-3 weeks
Amplification of recombinant baculovirus; generation of high-titer virus stock 1 week
Protein production 1 week
Purification 1-2 weeks
Total 5-9 weeks

D. Yeast System

Feature: Cost effective

Phases Time
Expression vector construction 1-2 weeks
Generation and selection of yeast colonies expressing target protein 1-2 weeks
Protein production 1 week
Purification 2-3 weeks
Total 5-8 weeks

Product Catalog

Service description Catalog # Price
Set-up fee (includes the cost for constructing the expression vector and testing the protein expression) GI-P100 US$ 500
Mammalian/Baculoviral System: 1mg GI-P110-1MG US$ 2800/mg
Mammalian/Baculoviral System: 1-5mg GI-P110-5MG US$ 2200/mg
Mammalian/Baculoviral System: 5-10mg GI-P110-10MG US$ 2000/mg
Mammalian/Baculoviral System: 10-20mg GI-P110-20MG US$ 1800/mg
Mammalian/Baculoviral System: >20mg GI-P110-C Please inquire
Bacterial/Yeast System: 1mg GI-P120-1MG US$ 2000/mg
Bacterial/Yeast System: 1mg GI-P120-1MG US$ 2000/mg
Bacterial/Yeast System: 1-5mg GI-P120-5MG US$ 1100/mg
Bacterial/Yeast System: 5-10mg GI-P120-10MG US$ 850/mg
Bacterial/Yeast System: 10-20mg GI-P120-20MG US$ 600/mg
Bacterial/Yeast System: >20mg GI-P120-C Please inquire

Guaranteed >95% purity for secreted protein and >75% purity for intracellular proteins

Contact us

If you like to purchase, or need any assistance, please do not hesitate to contact us at:

GeneImmune Biotechnology Corp.
15601 Crabbs Branch Way
E128
Rockville, MD 20855
Tel. 301-861-4366
Email. order@geneimmuneusa.com